Mass processing--an improved technique for protein identification with mass spectrometry data.

نویسندگان

  • Josh A Henkin
  • Mark E Jennings
  • Dwight E Matthews
  • Jim O Vigoreaux
چکیده

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis following tryptic digestion of polyacrylamide gel pieces is a common technique used to identify proteins. This approach is rapid, sensitive, and user friendly, and is becoming widely available to scientists in a variety of biological fields. Here we introduce a simple and effective strategy called "mass processing" where the list of masses generated from a mass spectrometer undergoes two stages of data reduction before identification. Mass processing improves the ability to identify in-gel tryptic-digested proteins by reducing the number of nonsample masses submitted to protein identification database search engines. Our results demonstrate that mass processing improves the statistical score and rank of putative protein identifications, especially with low-quantity samples, thus increasing the ability to confidently identify proteins with mass spectrometry data.

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عنوان ژورنال:
  • Journal of biomolecular techniques : JBT

دوره 15 4  شماره 

صفحات  -

تاریخ انتشار 2004